Debian Med Project
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Summary
Phylogeny
Debian Med phylogeny packages

This lists Debian packages related to phylogeny for use in life sciences. The purpose of this compilation of packages is to have a handy subset of from the med-bio metapackage which contains a lot more than only phylogeny related software.

Description

For a better overview of the project's availability as a Debian package, each head row has a color code according to this scheme:

If you discover a project which looks like a good candidate for Debian Med to you, or if you have prepared an unofficial Debian package, please do not hesitate to send a description of that project to the Debian Med mailing list

Links to other tasks

Debian Med Phylogeny packages

Official Debian packages with high relevance

Altree
Programm zur Phylogenie-basierten Assoziations- und Lokalisierungsanalyse
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ALTree wurde für entworfen, um Assoziationen zu entdecken und Suszeptibilitäts-Lokuse (susceptibility loci) mittels phylogenetischen Bäumen aus Haplotypen zu lokalisieren. Zum Einen ermöglicht sie die Entdeckung einer Assoziation zwischen einem untersuchten Gen und einer Krankheit, zum Anderen wird eine Hypothese über Suszeptibilitäts-Lokuse möglich.

Please cite: Claire Bardel, Vincent Danjean and Emmanuelle Genin: ALTree: association detection and localization of susceptibility sites using haplotype phylogenetic trees. (PubMed,eprint) Bioinformatics 22(11):1402-1403 (2006)
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Beast-mcmc
Bayesian MCMC phylogenetic inference
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BEAST is a cross-platform program for Bayesian MCMC analysis of molecular sequences. It is entirely orientated towards rooted, time-measured phylogenies inferred using strict or relaxed molecular clock models. It can be used as a method of reconstructing phylogenies but is also a framework for testing evolutionary hypotheses without conditioning on a single tree topology. BEAST uses MCMC to average over tree space, so that each tree is weighted proportional to its posterior probability. Included is a simple to use user-interface program for setting up standard analyses and a suit of programs for analysing the results.

The package is enhanced by the following packages: beast-mcmc-doc beast-mcmc-examples
Please cite: Alexei J Drummond and Andrew Rambaut: BEAST: Bayesian evolutionary analysis by sampling trees. (PubMed,eprint) BMC Evol Biol 8(7):214 (2007)
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Clustalw
Globale multiple Alignments von Nukleotid- oder Peptidsequenzen
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Clustal W führt ein Alignment mehrerer Nukleotid- oder Aminosäurensequenzen aus. Das Programm erkennt das Format der Eingabesequenzen und ob die Sequenzen aus Nuklein- (DNA/RNA) oder Aminosäuren (Proteine) bestehen. Das Ausgabeformat kann aus verschiedenen Formaten für multiple Alignments ausgewählt werden, etwa Phylip oder FASTA. Clustal W ist allgemein anerkannt.

Die Ausgabe von Clustal W kann händisch editiert werden, jedoch wird ein Alignment-Editor wie SeaView oder Clustal X empfohlen. Bei der Erstellung eines Modells aus Ihrem Alignment, kann die Ausgabe für verbesserte Datenbanksuchen verwendet werden. Das Debian-Paket hmmer erstellt dies mittels eines Hidden-Markov-Modells (HMM).

The package is enhanced by the following packages: clustalw-mpi
Please cite: M. A. Larkin, G. Blackshields, N. P. Brown, R. Chenna, P. A. McGettigan, H. McWilliam, F. Valentin, I.M. Wallace, A. Wilm, R. Lopez, J. D. Thompson, T. J. Gibson and D. G. Higgins: Clustal W and Clustal X version 2.0. (PubMed,eprint) Bioinformatics 23(21):2947-2948 (2007)
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Clustalx
Multiples Alignment von Nukleotid- und Proteinsequenzen (grafische Oberfläche)
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Dieses Paket enthält eine grafische Oberfläche für Clustal, ein Programm für multiple Sequenzalignments. Das Paket enthält für die Analyse der Resultate eine integrierte Umgebung für multiple Sequenz- und Profilalignments. Das Sequenzalignment wird in einem Fenster angezeigt. Es wurde ein vielseitiges Farbschema implementiert, um konservierte Teile des Alignments hervorzuheben. Für professionelle Präsentationen ist das LaTeX-Paket texshade oder boxshade besser geeignet.

Sie können alle Optionen für herkömmliche multiple Sequenz- und Profilaligments über Pull-Down-Menüs am oberen Rand des Fensters erreichen. Sie können Sequenzen ausschneiden und einfügen, um die Alignment-Reihenfole zu ändern; Sie können eine Untermenge an Sequenzen auswählen, die verglichen werden sollen; Sie können einen Teilbereich des Alignments auswählen, neu anordnen und wieder in das ursprüngliche Alignment einfügen.

Es kann eine Qualitätsanlayse des Alignments durchgeführt werden. Außerdem können Segmente mit geringem Score oder ungewöhnlichen Resten hervorgehoben werden.

Please cite: M.A. Larkin, G. Blackshields, N.P. Brown, R. Chenna, P.A. McGettigan, H. McWilliam, F. Valentin, I.M. Wallace, A. Wilm, R. Lopez, J.D. Thompson, T.J. Gibson and D.G. Higgins: Clustal W and Clustal X version 2.0. (PubMed,eprint) Bioinformatics 23(21):2947-2948 (2007)
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Dialign
Segmentbasiertes multiples Sequenzalignment
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Das Kommandozeilenwerkzeug DIALIGN2 führt ein multiples Alignment von Protein- oder DNA-Sequenzen aus. Es erstellt Alignments aus gap-freien Paaren von ähnlichen Sequenzsegmenten. Dieses Bewertungsverfahren ist der grundlegende Unterschied zwischen DIALIGN und anderen globalen oder lokalen Methoden für Sequenzalignments. Beachten Sie, dass DIALIGN bei Gaps das Ergebnis nicht schlechter bewertet.

Please cite: Burkhard Morgenstern: DIALIGN 2: improvement of the segment-to-segment approach to multiple sequence alignment. (PubMed,eprint) Bioinformatics 15(3):211-218 (1999)
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Dialign-tx
Segmentbasiertes multiples Sequenzalignment
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DIALIGN-TX ist ein Kommandozeilenwerkzeug, um mehrere Protein- oder DNA-Sequenzen aneinander auszurichten (»zu alignen«). Es handelt sich um eine vollständige Reimplementation des segmentbasierten Ansatzes, einschließlich einiger neuer Verbesserungen und Heuristiken und anderer Verbesserungen, die im Vergleich mit DIALIGN 2.2 oder DIALIGN-T deutlich die Qualität der ermittelten Alignments erhöht. Für paarweise Alignments nutzt DIALIGN-TX einen Algorithmus zur Verkettung von Fragmenten, der Ketten niedrig bewerteter lokaler Alignments den isolierten höher bewerteten Fragmenten vorzieht. Für multiple Alignments nutzt DIALIGN-TX eine verbesserte, erfolgshungrige Prozedur, die nicht auf falsche lokale Sequenzähnlichkeiten hereinfällt.

The package is enhanced by the following packages: dialign-tx-data
Please cite: Amarendran R. Subramanian, Michael Kaufmann and Burkhard Morgenstern: DIALIGN-TX: greedy and progressive approaches for segment-based multiple sequence alignment. (PubMed) Algorithms for Molecular Biology 3(1):6 (2008)
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Exonerate
Generisches Werkzeug zum paarweisen Sequenzvergleich
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Mit Exonerate können Sie Sequenzen ab- und vergleichen. Das Programm bietet Ihnen viele Alignment-Modelle, welche entweder vollständig dynamische Programmierung oder eine Reihe von heuristischen Methoden verwenden. Ein Großteil der Funktionalität der »Wise dynamic programming suite« wurde zur Erhöhung der Effizienz in C neu geschrieben. Exonerate ist eine wesentliche Komponente bei der Erstellung der »Ensembl Genome«-Datenbanken, die Ähnlichkeitsmaße für RNA- und DNA-Sequenzen bestimmt und so allgemein »splice variants« und Codesequenzen bestimmt.

Ein »In-silico PCR«-System zur Simulation von Experimenten (Siehe auch das Handbuch von »ipcress«) wird zusammen mit »exonerate« verteilt.

Dieses Paket beinhaltet auch eine Auswahl an Werkzeugen zur Erstellung einfacher, schneller Änderungen an »fasta«-Dateien mit über 2GB Dateigröße.

Please cite: Guy C. Slater and Ewan Birney: Automated generation of heuristics for biological sequence comparison. (PubMed,eprint) BMC Bioinformatics 6(1):31 (2005)
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Fastdnaml
Werkzeug zum Aufbau phylogenetischer Bäume von DNA-Sequenzen
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FastDNAml ist abgeleitet von Joseph Felsensteins DNAML Version 3.3 (Teil seines Pakets PHYLIP). Bevor Sie fastDNAml verwenden, sollten Sie die Dokumentation für DNAML heranziehen.

FastDNAml ist der Versuch, das gleiche Problem wie DNAML effektiver zu lösen, damit größere Bäume und/oder »bootstrap«-Replikate behandelt werden können. Bei großen Teilen von fastDNAml handelt es sich lediglich um eine Neuprogrammierung der in Pascal geschriebenen PHYLIP DNAML Version 3.3 in der Sprache C.

Beachten Sie, dass die Webseite dieses Programms nicht mehr verfügbar ist und dieses Programm daher wahrscheinlich nicht mehr aktualisiert wird.

Please cite: Gary J. Olsen, Hideo Matsuda, Ray Hagstrom and Ross Overbeek: fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. (PubMed,eprint) Comput Appl Biosci 10(1):41-48 (1994)
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Figtree
Grafischer Betrachter für phylogenetische Bäume
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FigTree ist gedacht als grafischer Betrachter für phylogenetische Bäume und als ein Programm, das veröffentlichungsreife Abbildungen erstellt. Insbesondere ist es für die Anzeige zusammengefasster und annotierter Bäume geeignet, die von BEAST erstellt wurden.

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Hmmer
Profil-Hidden-Markov-Modelle für Protein-Sequenzanalyse
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HMMER ist eine Implementierung der Profile-Hidden-Markov-Modelle für eine sensitive Suche in biologischen Sequenzdatenbanken mit multiplen Sequenzalignments als Anfragen.

Mit einem multiplen Sequenzalignment als Eingabe baut HMMER ein statistisches Modell, genannt »Hidden-Markov-Modell«. Mit diesem kann in Sequenzdatenbanken nach homologen Sequenzen gesucht werden. Dabei können gleichzeitig, der gesuchten Proteinfamilie zugehörige, Sequenzen alignt werden.

Please cite: S. R. Eddy: Profile hidden Markov models. (PubMed,eprint) Bioinformatics 14(9):755-763 (1998)
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Iqtree
efficient phylogenetic software by maximum likelihood
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IQ-TREE is a very efficient maximum likelihood phylogenetic software with following key features among others:

  • A novel fast and effective stochastic algorithm to estimate maximum likelihood trees. IQ-TREE outperforms both RAxML and PhyML in terms of likelihood while requiring similar amount of computing time (see Nguyen et al., 2015)
  • An ultrafast bootstrap approximation to assess branch supports (see Minh et al., 2013).
  • A wide range of substitution models for binary, DNA, protein, codon, and morphological alignments.
  • Ultrafast model selection for all data types, 10 to 100 times faster than jModelTest and ProtTest.
  • Finding best partition scheme like PartitionFinder.
  • Partitioned models with mixed data types for phylogenomic (multi- gene) alignments, allowing for separate, proportional, or joint branch lengths among genes.
  • Supporting the phylogenetic likelihod library (PLL) (see Flouri et al., 2014)
Please cite: Lam Tung Nguyen, Heiko A. Schmidt, Arndt von Haeseler and Bui Quang Minh: IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. (PubMed,eprint) Mol. Biol. Evol. 32(1):268-274 (2015)
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Jmodeltest
HPC selection of models of nucleotide substitution
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jModelTest is a tool to carry out statistical selection of best-fit models of nucleotide substitution. It implements five different model selection strategies: hierarchical and dynamical likelihood ratio tests (hLRT and dLRT), Akaike and Bayesian information criteria (AIC and BIC), and a decision theory method (DT). It also provides estimates of model selection uncertainty, parameter importances and model-averaged parameter estimates, including model-averaged tree topologies. jModelTest 2 includes High Performance Computing (HPC) capabilities and additional features like new strategies for tree optimization, model- averaged phylogenetic trees (both topology and branch length), heuristic filtering and automatic logging of user activity.

Please cite: Diego Darriba, Guillermo L Taboada, Ramón Doallo and David Posada: jModelTest 2: more models, new heuristics and parallel computing. (PubMed) Nature Methods 9(8):772 (2012)
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Kalign
Globales und fortschreitendes multiples Sequenzalignment
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Das Befehlszeilenwerkzeug Kalign gleicht mehrere biologische Sequenzen ab. Für die Erhöhung von Präzision und Geschwindigkeit nutzt es den Muth-Manber-Algorithmus, der in großen Datenmengen sich wiederholende Muster ermittelt. Das Programm verwendet einen globalen, fortschreitenden Alignment-Ansatz, ergänzt durch den Einsatz eines annähernden Musterabgleich-Algorithmus zur Berechnung von Sequenzabständen und durch die Einbeziehung lokaler Übereinstimmungen in ein ansonsten globales Alignment.

Please cite: Timo Lassmann, Oliver Frings and Erik L. L. Sonnhammer: Kalign2: high-performance multiple alignment of protein and nucleotide sequences allowing external features. (PubMed,eprint) Nucl. Acids Res. 37(3):858-865 (2009)
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Mrbayes
Bayessche Inferenz der Phylogenie
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Die Bayessche Inferenz der Phylogenie basiert auf einer Quantität - A-posteriori-Wahrscheinlichkeitsverteilung von Bäumen genannt - die die Wahrscheinlichkeit eines Baumes bedingt durch die Beobachtungen ist. Die Bedingung entsteht durch Verwendung des Bayestheorems. Die A-posteriori-Wahrscheinlichkeitsverteilung von Bäumen kann unmöglich analytisch berechnet werden. Stattdessen verwendet MrBayes eine Simulation, das Markov-Chain-Monte-Carlo-Verfahren (kurz MCMC), um die A-posteriori-Wahrscheinlichkeiten der Bäume zu schätzen.

The package is enhanced by the following packages: mrbayes-doc
Please cite: Fredrik Ronquist, Maxim Teslenko, Paul van der Mark, Daniel L. Ayres, Aaron Darling, Sebastian Höhna, Bret Larget, Liang Liu, Marc A. Suchard and John P. Huelsenbeck: MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice across a Large Model Space. (PubMed,eprint) Systematic Biology (2012)
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Muscle
Programm zur Ausrichtung mehrerer Proteinsequenzen
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MUSCLE (multiple sequence comparison by log-expectation) ist ein Programm für die Ausrichtung mehrerer Proteinsequenzen. In der durch den Autor durchgeführten Vergleichsstudie erreichte MUSCLE die höchste Bewertung aller getesteten Programme in mehreren Benchmarks der Genauigkeit der Ausrichtungen - und war dabei eines der schnellsten.

Please cite: Robert C. Edgar: MUSCLE: multiple sequence alignment with high accuracy and high throughput. (PubMed,eprint) Nucleic Acids Research 32(5):1792-1797 (2004)
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Mustang
Struktur-Alignment mehrerer Proteine
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Mustang ist ein Algorithmus, um mehrere Protein-Strukturen miteinander abzugleichen. Aus einem Satz von PDB-Dateien (Protein Data Bank, PDB) bestimmt das Programm unter Ausnutzung der räumlichen Informationen in den Calpha-Atomen die einander zuzuordnenden Aminosäuren. Dies ist für verschiedene Bereiche des Proteins auch unterschiedlich gut möglich. Anschließend werden die Proteine paarweise miteinander verglichen, und so in mehreren Durchgängen mit einer Heuristik das Ergebnis zunehmend verfeinert. Schließlich gibt Mustang die Zuordnung der Protein-Sequenzen (das Alignment) und auch die entsprechende räumliche Überlagerung von Strukturen aus.

The package is enhanced by the following packages: mustang-testdata
Please cite: Arun S. Konagurthu, James C. Whisstock, Peter J. Stuckey and Arthur M. Lesk: MUSTANG: A multiple structural alignment algorithm. (PubMed) Proteins: Structure, Function, and Bioinformatics 64(3):559-574 (2006)
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Njplot
Programm zur Darstellung phylogenetischer Bäume
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NJplot kann alle Dendrogramme zeichnen, die im Newick-Format notiert sind. Dieses Format ist das Standardformat für phylogenetische Bäume (und wird z.B. vom Phylip-Paket verwendet). NJplot ist besonders praktisch, um mit den Baumerstellungsmethoden parsimony, distance oder maximum-likelihood erzeugte ungewurzelte Bäume in gewurzelte Bäume zu überführen.

Please cite: G. Perrière and M. Gouy: WWW-query: An on-line retrieval system for biological sequence banks. (PubMed) Biochimie 78(5):364–369 (1996)
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Phylip
package of programs for inferring phylogenies
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The PHYLogeny Inference Package is a package of programs for inferring phylogenies (evolutionary trees) from sequences. Methods that are available in the package include parsimony, distance matrix, and likelihood methods, including bootstrapping and consensus trees. Data types that can be handled include molecular sequences, gene frequencies, restriction sites, distance matrices, and 0/1 discrete characters.

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Phyml
Phylogenetic estimation using Maximum Likelihood
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PhyML is a software that estimates maximum likelihood phylogenies from alignments of nucleotide or amino acid sequences. It provides a wide range of options that were designed to facilitate standard phylogenetic analyses. The main strengths of PhyML lies in the large number of substitution models coupled to various options to search the space of phylogenetic tree topologies, going from very fast and efficient methods to slower but generally more accurate approaches. It also implements two methods to evaluate branch supports in a sound statistical framework (the non-parametric bootstrap and the approximate likelihood ratio test).

PhyML was designed to process moderate to large data sets. In theory, alignments with up to 4,000 sequences 2,000,000 character-long can be analyzed. In practice however, the amount of memory required to process a data set is proportional of the product of the number of sequences by their length. Hence, a large number of sequences can only be processed provided that they are short. Also, PhyML can handle long sequences provided that they are not numerous. With most standard personal computers, the “comfort zone” for PhyML generally lies around 3 to 500 sequences less than 2,000 character long.

This package also includes PhyTime.

Please cite: Stéphane Guindon: Bayesian estimation of divergence times from large sequence alignments. (PubMed,eprint) Molecular Biology and Evolution 27(8):1768-81 (2010)
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Poa
Partial Order Alignment for multiple sequence alignment
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POA is Partial Order Alignment, a fast program for multiple sequence alignment (MSA) in bioinformatics. Its advantages are speed, scalability, sensitivity, and the superior ability to handle branching / indels in the alignment. Partial order alignment is an approach to MSA, which can be combined with existing methods such as progressive alignment. POA optimally aligns a pair of MSAs and which therefore can be applied directly to progressive alignment methods such as CLUSTAL. For large alignments, Progressive POA is 10-30 times faster than CLUSTALW.

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Populations
population genetic software
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Populations is a population genetic software. It computes genetic distances between populations or individuals. It builds phylogenetic trees (NJ or UPGMA) with bootstrap values.

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Proalign
Probabilistic multiple alignment program
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ProAlign performs probabilistic sequence alignments using hidden Markov models (HMM). It includes a graphical interface (GUI) allowing to (i) perform alignments of nucleotide or amino-acid sequences, (ii) view the quality of solutions, (iii) filter the unreliable alignment regions and (iv) export alignments to other software.

ProAlign uses a progressive method, such that multiple alignment is created stepwise by performing pairwise alignments in the nodes of a guide tree. Sequences are described with vectors of character probabilities, and each pairwise alignment reconstructs the ancestral (parent) sequence by computing the probabilities of different characters according to an evolutionary model.

Please cite: Ari Löytynoja and Michel C Milinkovitch: A hidden Markov model for progressive multiple alignment. (PubMed,eprint) Bioinformatics 19(12):1505-13 (2003)
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Probalign
multiple sequence alignment using partition function posterior probabilities
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Probalign uses partition function posterior probability estimates to compute maximum expected accuracy multiple sequence alignments. It performs statistically significantly better than the leading alignment programs Probcons v1.1, MAFFT v5.851, and MUSCLE v3.6 on BAliBASE 3.0, HOMSTRAD, and OXBENCH benchmarks. Probalign improvements are largest on datasets containing N/C terminal extensions and on datasets with long and heterogeneous length sequences. On heteregeneous length datasets containing repeats Probalign alignment accuracy is 10% and 15% higher than the other three methods when standard deviation of length is at least 300 and 400.

Please cite: Usman Roshan and Dennis R. Livesay: Probalign: multiple sequence alignment using partition function posterior probabilities. (PubMed,eprint) Bioinformatics 22(22):2715-21 (2006)
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Probcons
PROBabilistic CONSistency-based multiple sequence alignment
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Tool for generating multiple alignments of protein sequences. Using a combination of probabilistic modeling and consistency-based alignment techniques, PROBCONS has achieved the highest accuracies of all alignment methods to date. On the BAliBASE benchmark alignment database, alignments produced by PROBCONS show statistically significant improvement over current programs, containing an average of 7% more correctly aligned columns than those of T-Coffee, 11% more correctly aligned columns than those of CLUSTAL W, and 14% more correctly aligned columns than those of DIALIGN.

Please cite: Chuong B. Do, Mahathi S.P. Mahabhashyam, Michael Brudno and Serafim Batzoglou: ProbCons: Probabilistic consistency-based multiple sequence alignment. (PubMed,eprint) Genome Research 15(2):330-340 (2005)
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Proda
Mulitple Alignments von Proteinsequenzen
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ProDA ist ein System zur automatischen Erkennung und Anordnung von homologen Regionen in Proteinsammlungen mit beliebigen Domain-Architekturen. Bei gegebenem Eingangssatz von nicht-angeordneten Sequenzen identifiziert ProDA eine homologe Region, die in einer oder mehrere Sequenzen auftaucht und liefert eine Sammlung von lokalen multiplen Anordnungen für diese Regionen zurück.

Please cite: Tu Minh Phuong, Chuong B. Do, Robert C. Edgar and Serafim Batzoglou: Multiple alignment of protein sequences with repeats and rearrangements. (PubMed,eprint) Nucl. Acids Res. 34(20):5932-5942 (2006)
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Prottest
selection of best-fit models of protein evolution
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PROTTEST (ModelTest's relative) is a program for selecting the model of protein evolution that best fits a given set of sequences (alignment). This java program is based on the Phyml program (for maximum likelihood calculations and optimization of parameters) and uses the PAL library as well. Models included are empirical substitution matrices (such as WAG, LG, mtREV, Dayhoff, DCMut, JTT, VT, Blosum62, CpREV, RtREV, MtMam, MtArt, HIVb, and HIVw) that indicate relative rates of amino acid replacement, and specific improvements (+I:invariable sites, +G: rate heterogeneity among sites, +F: observed amino acid frequencies) to account for the evolutionary constraints impossed by conservation of protein structure and function. ProtTest uses the Akaike Information Criterion (AIC) and other statistics (AICc and BIC) to find which of the candidate models best fits the data at hand.

Please cite: Diego Darriba, Guillermo L. Taboada, Ramón Doallo and David Posada: ProtTest 3: fast selection of best-fit models of protein evolution. (PubMed,eprint) Bioinformatics 27(8):1164-5 (2011)
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Seaview
Schnittstelle mehrerer Plattformen für Sequenzalignments und Phylogenie
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SeaView liest und schreibt unterschiedliche Dateiformate (NEXUS, MSF, CLUSTAL, FASTA, PHYLIP, MASE, Newick) von DNA- und Proteinsequenzen sowie von phylogenetischen Bäumen. Alignments können manuell bearbeitet werden. Mittels SeaView können die Programme Muscle oder Clustal Omega multiple Sequenzalignments durchführen. Es erlaubt ebenfalls beliebige externe Alignment-Algorithmen zu verwenden, die FASTA-formatierte Dateien lesen und schreiben können. SeaView berechnet phylogenetische Bäume mit Parsinomie mittels PHYLIPs Algorithmus dnapars/protpars, per Distanz mit den Algorithmen NJ oder NioNJ mit einer Vielzahl an evolutionären Distanzen oder per Maximum-Likelihood mittels dem Programm PhyML 3.0. SeaView zeichnet phylogenetische Bäume auf dem Bildschirm oder in PostScript-Dateien und ermöglicht den Download von Sequenzen über das Internet bei EMBL/GenBank/UniProt.

The package is enhanced by the following packages: muscle
Please cite: Manolo Gouy, Stephane Guindon and Olivier Gascuel: SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. (PubMed,eprint) Mol Biol Evol 27(2):221-224 (2010)
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Sigma-align
Einfache multiple Alignments nicht-kodierender DNA-Sequenzen
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Sigma (»Simple greedy multiple alignment«) ist ein Alignment-Programm. Der Algorithmus und das Scoring-Schema sind speziell für nicht-kodierende DNA-Sequenzen entworfen.

Das Programm verwendet die Strategie des Suchens der bestmöglichen lückenlosen lokalen Alignments. Dies passiert bei jedem Schritt indem ein bestmögliches Alignment passend zu existierenden Alignments ermittelt wird. Sigma bewertet die Signifikanz der Alignments basierend auf den Längen der alignierten Fragmente und eines Hintergrundmodells. Diese können über eine Hilfsdatei intergenischer DNA zur Verfügung gestellt oder geschätzt werden.

Please cite: Siddharthan, Rahul: Sigma: multiple alignment of weakly-conserved non-coding DNA sequence. (PubMed) BMC Bioinformatics 7(1):143 (2006)
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Spread-phy
analyze and visualize phylogeographic reconstructions
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SPREAD is a user-friendly application to analyze and visualize phylogeographic reconstructions resulting from Bayesian inference of spatio-temporal diffusion.

There is a tutorial for SPREAD online at http://www.kuleuven.be/aidslab/phylogeography/tutorial/spread_tutorial.html

Originally this program is named "spread". However, there is just such a package inside Debian and thus a 'phy' for phylogeny was prepended.

Please cite: Filip Bielejec, Andrew Rambaut, Marc A. Suchard and Philippe Lemey: SPREAD: spatial phylogenetic reconstruction of evolutionary dynamics. (PubMed,eprint) Bioinformatics 27(20):2910-2912 (2011)
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T-coffee
Multiple Sequence Alignment
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T-Coffee is a multiple sequence alignment package. Given a set of sequences (Proteins or DNA), T-Coffee generates a multiple sequence alignment. Version 2.00 and higher can mix sequences and structures.

T-Coffee allows the combination of a collection of multiple/pairwise, global or local alignments into a single model. It can also estimate the level of consistency of each position within the new alignment with the rest of the alignments. See the pre-print for more information

T-Coffee has a special called M-Coffee that makes it possible to combine the output of many multiple sequence alignment packages. In its published version, it uses MUSCLE, PROBCONS, POA, DiAlign-TS, MAFFT, Clustal W, PCMA and T-Coffee. A special version has been made for Debian, DM-Coffee, that uses only free software by replacing Clustal W by Kalign. Using the 8 Methods of M-Coffee can sometimes be a bit heavy. You can use a subset of your favorite methods if you prefer.

The package is enhanced by the following packages: clustalw dialign-tx kalign mafft muscle ncbi-blast+ poa prank probcons tm-align
Please cite: Cédric Notredame, Desmond G. Higgins and Jaap Heringa: T-coffee: a novel method for fast and accurate multiple sequence alignment. (PubMed) Journal of Molecular Biology 302(1):205-217 (2000)
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Tm-align
structural alignment of proteins
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TM-align is a computer algorithm for protein structure alignment using dynamic programming. The scoring is performed by the TM-score rotation matrix. This is similar to the RMSD in that unaligned portions of the structure influence the scoring less than the more structurally conserved regions.

Please cite: Yang Zhang and Jeffrey Skolnick: TM-align: A protein structure alignment algorithm based on TM-score. (PubMed,eprint) Nucleic Acids Research 33(7):2302-2309 (2005)
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Tree-ppuzzle
Parallelisierte Rekonstruktion phylogenetischer Bäume durch »Maximum Likelihood«
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TREE-PUZZLE (früher PUZZLE) ist ein interaktives Konsolenprogramm, das einen schnellen Suchalgorithmus über einen Baum, sogenanntes »Quartet Puzzling«, implementiert. Es erlaubt die Analyse großer Datenmengen und trifft automatisch Entscheidungen über die Anordnung der jeweiligen Verzweigungen. Weiterhin berechnet TREE-PUZZLE mittels der paarweisen Maximum-Likelihood-Methode Abstände und Längen von Verzweigungen für vom Benutzer definierte Bäume. Die Längen der Verzweigungen können auch unter der »Clock-Assumption« berechnet werden. Außerdem bietet TREE-PUZZLE eine neue Methode, das »Likelihood Mapping«, um die Anordnung einer hypothetischen internen Verzweigung zu untersuchen, ohne den gesamten Baum zu berechnen und um den phylogenetischen Zusammenhang eines Sequenzalignments anzuzeigen.

Dies ist eine parallelisierte Version von tree-puzzle.

Please cite: Heiko A. Schmidt, Korbinian Strimmer, Martin Vingron and Arndt von Haeseler: TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing. (PubMed,eprint) Bioinformatics 18(3):502-504 (2002)
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Tree-puzzle
Rekonstruktion phylogenetischer Bäume durch »Maximum Likelihood«
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TREE-PUZZLE (früher PUZZLE) ist ein interaktives Konsolenprogramm, das einen schnellen Suchalgorithmus über einen Baum, sogenanntes »Quartet Puzzling«, implementiert. Es erlaubt die Analyse großer Datenmengen und trifft automatisch Entscheidungen über die Anordnung der jeweiligen Verzweigungen. Weiterhin berechnet TREE-PUZZLE mittels der paarweisen Maximum-Likelihood-Methode Abstände und Längen von Verzweigungen für vom Benutzer definierte Bäume. Die Längen der Verzweigungen können auch unter der »Clock-Assumption« berechnet werden. Außerdem bietet TREE-PUZZLE eine neue Methode, das »Likelihood Mapping«, um die Anordnung einer hypothetischen internen Verzweigung zu untersuchen, ohne den gesamten Baum zu berechnen und um den phylogenetischen Zusammenhang eines Sequenzalignments anzuzeigen.

Please cite: Heiko A. Schmidt, Korbinian Strimmer, Martin Vingron and Arndt von Haeseler: TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing. (PubMed,eprint) Bioinformatics 18(3):502-504 (2002)
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Treeview
Java re-implementation of Michael Eisen's TreeView
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TreeView creates a matrix-like display of expression data, known as Eisen clustering. The original implementation was a Windows program named TreeView by Michael Eisen. This TreeView package, sometimes also referred to as jTreeView, was rewritten in Java under a free license.

Java TreeView is an extensible viewer for microarray data in PCL or CDT format.

Please cite: Alok J. Saldanha: Java Treeview -- extensible visualization of microarray data. (PubMed,eprint) Bioinformatics 20(17):3246-3248 (2004)
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Treeviewx
Anzeige und Druck phylogenetischer Bäume
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TreeViewX ist ein für mehrere Plattformen verfügbares Programm zur Darstellung phylogenetischer Bäume. Es kann Bäume in den Formaten NEXUS und Newick lesen und darstellen (so wie die, die von PAUP*, ClustalX, TREE-PUZZLE und anderen Programmen ausgegeben werden). Es ermöglicht das Anordnen der Zweige der Bäume und den Export der Bäume in das SVG-Format.

Please cite: Page, Roderic D. M.: TreeView: an application to display phylogenetic trees on personal computers. (PubMed) Comput. Appl. Biosci. 12(4):357-8 (1996)
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Official Debian packages with lower relevance

Python3-treetime
inference of time stamped phylogenies and ancestral reconstruction (Python 3)
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TreeTime provides routines for ancestral sequence reconstruction and the maximum likelihoo inference of molecular-clock phylogenies, i.e., a tree where all branches are scaled such that the locations of terminal nodes correspond to their sampling times and internal nodes are placed at the most likely time of divergence.

TreeTime aims at striking a compromise between sophisticated probabilistic models of evolution and fast heuristics. It implements GTR models of ancestral inference and branch length optimization, but takes the tree topology as given. To optimize the likelihood of time-scaled phylogenies, treetime uses an iterative approach that first infers ancestral sequences given the branch length of the tree, then optimizes the positions of unconstraine d nodes on the time axis, and then repeats this cycle. The only topology optimization are (optional) resolution of polytomies in a way that is most (approximately) consistent with the sampling time constraints on the tree. The package is designed to be used as a stand-alone tool or as a library used in larger phylogenetic analysis workflows.

Features

  • ancestral sequence reconstruction (marginal and joint maximum likelihood)
  • molecular clock tree inference (marginal and joint maximum likelihood)
  • inference of GTR models
  • rerooting to obtain best root-to-tip regression
  • auto-correlated relaxed molecular clock (with normal prior)

This package provides the Python 3 module.

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Debian packages in contrib or non-free

Gmap
spliced and SNP-tolerant alignment for mRNA and short reads
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This package contains the programs GMAP and GSNAP as well as utilities to manage genome databases in GMAP/GSNAP format. GMAP (Genomic Mapping and Alignment Program) is a tool for aligning EST, mRNA and cDNA sequences. GSNAP (Genomic Short-read Nucleotide Alignment Program) is a tool for aligning single-end and paired-end transcriptome reads. Both tools can use a database of

  • known splice sites and identify novel splice sites.
  • known single-nucleotide polymorphisms (SNPs). GSNAP can align bisulfite-treated DNA.
Please cite: Thomas D. Wu and Colin K. Watanabe: GMAP: a genomic mapping and alignment program for mRNA and EST sequences. (eprint) Bioinformatics 21(9):1859-1875 (2005)
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Packaging has started and developers might try the packaging code in VCS

Forester
Graphical vizualiation tool Archaeopteryx
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Archaeopteryx is a software tool for the visualization, analysis, and editing of potentially large and highly annotated phylogenetic trees. It can be used both as applet (ArchaeopteryxA and ArchaeopteryxE) and as a standalone application.

Patristic
Calculate patristic distances and comparing the components of genetic change
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Patristic overcomes some logistic barriers to analysing signals in sequences. In additional to calculating patristic distances, it provides plots for any combination of matrices, calculates commonly used statistics, allows data such as isolation dates to be entered and reorders matrices with matching species or gene labels. It will be used to analyse rates of mutation and substitutional saturation and the evolution of viruses.

Please cite: Mathieu Fourment and Mark J Gibbs: PATRISTIC: a program for calculating patristic distances and graphically comparing the components of genetic change. (PubMed,eprint) BMC Evolutionary Biology 6:1 (2006)

No known packages available but some record of interest (WNPP bug)

Phylowin - wnpp
Graphical interface for molecular phylogenetic inference
License: unknown
Debian package not available

Phylo_win is a graphical colour interface for molecular phylogenetic inference. It performs neighbor-joining, parsimony and maximum likelihood methods and bootstrap with any of them. Many distances can be used including Jukes & Cantor, Kimura, Tajima & Nei, HKY, Galtier & Gouy (1995), LogDet for nucleotidic sequences, Poisson correction for protein sequences, Ka and Ks for codon sequences. Species and sites to include in the analysis are selected by mouse. Reconstructed trees can be drawn, edited, printed, stored and evaluated according to numerous criteria.

This program uses sources files from the Phylip program, which forbids its use for profit. Therfore, Phylo_win will unfortunately have to be distributed in contrib or non-free.

Remark of Debian Med team: Issuer of previous ITP said:

Because I could never figure out the license of Phylo_win, and because the upstream authors released SeaView 4, which provides similar functionalities, I will not package Phylo_win.

Probably it makes sense to remove this project from the prospective packages list.

No known packages available

Gbioseq
DNA sequence editor for Linux
License: GPL
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gBioSeq is in an early stage of development, but it is already running. The goal is to provide an easy to use software to edit DNA sequences under Linux, Windows, MacOsX, using GTK C# (Mono).

Jstreeview
Editor for Phylogenetic Trees
License: MIT/X11
Debian package not available
Language: JavaScript

A concise viewer/editor for phylogenetic trees in the Newick format. The core functions are written in JavaScript, using the canvas tag proposed by HTML 5. No server side support is needed for rendering the picture and therefore you can grab this page together with knhx.js and canvastext.js to locally view your trees in a supported web browser.

The source can be downloaded at http://www.sanger.ac.uk/Users/lh3/download/jstreeview.zip

Phpphylotree
draw phylogenetic trees
License: GPL
Debian package not available

PhpPhylotree is a web application that is able to draw phylogenetic trees. It produces an SVG (Scalable Vector Graphic) file from phylip/newick tree files.

Treetime
Bayesian sampling of phylogenetic trees from molecular data
License: GPL
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TreeTime is controlled by input files in nexus format and does bayesian sampling of phylogenetic trees from these data.

*Popularitycontest results: number of people who use this package regularly (number of people who upgraded this package recently) out of 200201